Arzneimitteleinsatz zur Steuerung des Reproduktionsgeschehens in der biolo-gischen Ferkelproduktion

Under the rules of the EU Regulation 2092/91 “…the use of hormones or similar substances to control reproduction (e.g. induction or synchronisation of oestrus), or for other purposes, is prohibited. Nevertheless, hormones may be administered to an individual animal, as a form of therapeutic veterinary treatment.” This article tries to explain the difference between veterinary treatment and zootechnic measures in organic pig production such as synchronisation of oestrus and shows critical control points for the inspection body to improve the quality of inspection visits

Major Chemical Elements in Soot and Particulate Matter Exhaust Emissions Generated from In-Use Diesel Engine Passenger Vehicles

In this research we apply a sensitive laser optical technique for the measurement of main chemical elements present in the exhaust emissions generated from different in-use Diesel engine passenger vehicles. We use the laser-induced breakdown spectroscopy (LIBS) technique for diagnostics of miscellaneous Diesel particulate matter (DPM) formed from combustion Diesel engine exhaust emissions. Here we analysed particulate matter (PM) extracted from exhaust manifold part, from 67 different passenger vehicles of major brands from European car producers, that are used in daily life environment. The aim of this study is to develop LIBS technique for measurement of PM and to compare the emission matrix composition and major chemical elements within the Diesel particulate matter from exhaust manifold part. The presence of these elements in PM is linked with various processes inside the Diesel combustion engine

Identification of the Minor Chemical Elements in the Particulate Matter Exhaust Emissions From In-Use Diesel Engine Passenger Vehicles

In this research, we investigate the minor chemical elements contained in the particulate matter (PM) exhaust emissions, generated by in-use diesel engine passenger vehicles. For this purpose, we apply a high-resolution optical emission spectroscopy technique, for precise, spectrochemical analysis of diesel particulate matter (DPM). By means of the laser-induced breakdown spectroscopy analytical method, we analyse PM from different road diesel engine vehicles. DPM were obtained from miscellaneous in-use diesel engine passenger vehicles of diverse types and models from major brand car producers in Europe. We analysed particulate matter extracted from the exhaust manifold part, from 67 different passenger vehicles, which are used in daily life environment

Beifütterung von Ferkeln in freien Haltungssystemen

The digestive capacity of a piglet and the enzyme activity in the first weeks are aimed corresponding to the piglets requirements. Early provision of creep feed is necessary. The aim of the project was to study the feeding behaviour of piglets and sows during lactation in three different housing systems. In total data of 93 litters and 917 piglets were evaluated. The sows farrowed in either the Welser pen or the WelCon pen. They either remained in these systems until the end of lactation, or changed after 14 days to a multi-suckling system. The sows were fed ad libitum. Feed consumption was recorded weekly. The creep feeding started when piglet´s age reached 17 days on average. The feed supply for the piglets was dry and on the ground. Weight gain and feed consumption of piglets and sows were examined. In order to determine the length of stay of piglets and sows at the feeding place, video observation were performed. Only in the last suckling week the feed consumption of the piglets increased. Significant differences between the Welser pen, WelCon pen and multi-suckling system were found

Intracellular Ca2+ Inhibits Smooth Muscle L-Type Ca2+ Channels by Activation of Protein Phosphatase Type 2B and by Direct Interaction with the Channel

Modulation of L-type Ca2+ channels by tonic elevation of cytoplasmic Ca2+ was investigated in intact cells and inside-out patches from human umbilical vein smooth muscle. Ba2+ was used as charge carrier, and run down of Ca2+ channel activity in inside-out patches was prevented with calpastatin plus ATP. Increasing cytoplasmic Ca2+ in intact cells by elevation of extracellular Ca2+ in the presence of the ionophore A23187 inhibited the activity of L-type Ca2+ channels in cell-attached patches. Measurement of the actual level of intracellular free Ca2+ with fura-2 revealed a 50% inhibitory concentration (IC50) of 260 nM and a Hill coefficient close to 4 for Ca2+- dependent inhibition. Ca2+-induced inhibition of Ca2+ channel activity in intact cells was due to a reduction of channel open probability and availability. Ca2+-induced inhibition was not affected by the protein kinase inhibitor H-7 (10 μM) or the cytoskeleton disruptive agent cytochalasin B (20 μM), but prevented by cyclosporin A (1 μg/ ml), an inhibitor of protein phosphatase 2B (calcineurin). Elevation of Ca2+ at the cytoplasmic side of inside-out patches inhibited Ca2+ channels with an IC50 of 2 μM and a Hill coefficient close to unity. Direct Ca2+-dependent inhibition in cell-free patches was due to a reduction of open probability, whereas availability was barely affected. Application of purified protein phosphatase 2B (12 U/ml) to the cytoplasmic side of inside-out patches at a free Ca2+ concentration of 1 μM inhibited Ca2+ channel open probability and availability. Elevation of cytoplasmic Ca2+ in the presence of PP2B, suppressed channel activity in inside-out patches with an IC50 of ∼380 nM and a Hill coefficient of ∼3; i.e., characteristics reminiscent of the Ca2+ sensitivity of Ca2+ channels in intact cells. Our results suggest that L-type Ca2+ channels of smooth muscle are controlled by two Ca2+-dependent negative feedback mechanisms. These mechanisms are based on (a) a protein phosphatase 2B-mediated dephosphorylation process, and (b) the interaction of intracellular Ca2+ with a single membrane-associated site that may reside on the channel protein itself

The function of 7D-cadherins: a mathematical model predicts physiological importance for water transport through simple epithelia

<p>Abstract</p> <p>Background</p> <p>7D-cadherins like LI-cadherin are cell adhesion molecules and represent exceptional members of the cadherin superfamily. Although LI-cadherin was shown to act as a functional Ca²⁺-dependent adhesion molecule, linking neighboring cells together, and to be dysregulated in a variety of diseases, the physiological role is still enigmatic. Interestingly 7D-cadherins occur only in the lateral plasma membranes of cells from epithelia of water transporting tissues like the gut, the liver or the kidney. Furthermore LI-cadherin was shown to exhibit a highly cooperative Ca²⁺-dependency of the binding activity. Thus it is tempting to assume that LI-cadherin regulates the water transport through the epithelium in a passive fashion by changing its binding activity in dependence on the extracellular Ca²⁺.</p> <p>Results</p> <p>We developed a simple mathematical model describing the epithelial lining of a lumen with a content of variable osmolarity covering an interstitium of constant osmolarity. The width of the lateral intercellular cleft was found to influence the water transport significantly. In the case of hypertonic luminal content a narrow cleft is necessary to further increase concentration of the luminal content. If the cleft is too wide, the water flux will change direction and water is transported into the lumen. Electron microscopic images show that in fact areas of the gut can be found where the lateral intercellular cleft is narrow throughout the lateral cell border whereas in other areas the lateral intercellular cleft is widened.</p> <p>Conclusions</p> <p>Our simple model clearly predicts that changes of the width of the lateral intercellular cleft can regulate the direction and efficiency of water transport through a simple epithelium. In a narrow cleft the cells can increase the concentration of osmotic active substances easily by active transport whereas if the cleft is wide, friction is reduced but the cells can hardly build up high osmotic gradients. It is now tempting to speculate that 7D-cadherins, owing to their location and their Ca²⁺-dependence, will adapt their binding activity and thereby the width of the lateral intercellular cleft automatically as the Ca²⁺-concentration is coupled to the overall electrolyte concentration in the lateral intercellular cleft. This could provide a way to regulate the water resorption in a passive manner adapting to different osmotic conditions.</p

Entanglement witnesses and geometry of entanglement of two--qutrit states

We construct entanglement witnesses with regard to the geometric structure of the Hilbert--Schmidt space and investigate the geometry of entanglement. In particular, for a two--parameter family of two--qutrit states that are part of the magic simplex we calculate the Hilbert--Schmidt measure of entanglement. We present a method to detect bound entanglement which is illustrated for a three--parameter family of states. In this way we discover new regions of bound entangled states. Furthermore we outline how to use our method to distinguish entangled from separable states.Comment: 23 pages, 8 figure